Correctness and accuracy of template-based modeled single chain fragment variable (scFv) protein anti-breast cancer cell line (MCF-7)
نویسندگان
چکیده
منابع مشابه
Generation and immune-characterization of single chain fragment variable (scFv) antibody recognize breast cancer cells line (MCF-7)
Phage display technologies were used to produce singlechain antibodies (scFv) against breast cancer cell line (MCF-7). The mouse B cell hybridoma line C3A8 was the starting material, which generates a monoclonal antibody against breast cancer cells (MCF-7). The best candidate scFv sequences, based on (ELISA) screening data were sub-cloned into HB2151 host strain. The scFv gene was expressed in ...
متن کاملConstruction of single-chain variable fragment antibodies against MCF-7 breast cancer cells.
A phage display library of single chain variable fragment (scFv) against MCF-7 breast cancer cells was constructed from C3A8 hybridoma cells. RNA from the C3A8 was isolated, cDNA was constructed, and variable heavy and light immunoglobulin chain gene region were amplified using PCR. The variable heavy and light chain gene regions were combined with flexible linker, linked to a pCANTAB 5E phagem...
متن کاملInvestigating the antioxidant and anti-proliferative activity of Foeniculum vulgare seed essential oil on MCF-7 breast cancer cell line and A2780 ovarian cancer cell line
Background & aim: Due to the presence of high amounts of compounds with antioxidant and cytotoxic properties in Foeniculum vulgare mill seed essential oil, the aim of this study was to investigate the toxicity of Foeniculum vulgare mill seed essential oil on MCF-7 breast cancer cell line and A2780 ovarian cancer cell line and to investigate the antioxidant activity of the essential oil of this ...
متن کاملAnti-tumor Effects of Essential Oils of Red Clover and Ragweed on MCF-7 Breast Cancer Cell Line
Introduction: Breast cancer is one of the most common malignancies among Iranian women. Nowadays, the use of traditional medicinal plants has emerged as a tempting complementary to the treatment of breast cancer due to minimal side effects and less documented drug resistance. The aim of this study was to examine the anticancer effects of the essential oils of red clover and mature fruit of Bass...
متن کاملAnti-tumor Effects of Essential Oils of Red Clover and Ragweed on MCF-7 Breast Cancer Cell Line
Introduction: Breast cancer is one of the most common malignancies among Iranian women. Nowadays, the use of traditional medicinal plants has emerged as a tempting complementary to the treatment of breast cancer due to minimal side effects and less documented drug resistance. The aim of this study was to examine the anticancer effects of the essential oils of red clover and mature fruit of Bass...
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ژورنال
عنوان ژورنال: Open Journal of Genetics
سال: 2013
ISSN: 2162-4453,2162-4461
DOI: 10.4236/ojgen.2013.33021